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Whey lactose bioconversion in high-added value products using cheep renewable substrate

Oligolac is a project aim to revalorization of renewable substrate of dairy industry such as whey transforming it into bioproducts that may be used in zootechny field as feed additives with immune modulatory and prebiotic effect. The project will diversify protein and mineral sources in animals diet

The goal of the present project is to produce single cell biomass, oligosaccharides, known as Bifidus growth factor, and microelement chelates of amino acids from adapted, selected and high productively lactic yeast grown on a low cost substrate, such as cheese whey, by modification of the biotechnological conditions. In present, for maximization of oligosaccharides and lactic yeast biomass production it was used industrial lactose (expansive to produce) but is important to know the behavior of lactic yeast strains on whole liquid sweet whey as source of lactose. These products will be use as prebiotic for new feed additives for animal total mixed ratio feed. Now is used as feed additive Saccharomyces cerevisiae biomass grown on molasses residues. Functional supplements derived from lactic yeast biomass modulate the immune system in animals, contribute to significative improvement of weight gain (up to 15%), maintain the proper balance of microbes in the gastrointestinal tract, reducing the frequency of germs like Salmonella, E. coli, Clostridium in the intestine, reducing veterinary costs, and enhance nutrient utilization, decrease intestinal disease, prevent early mortality, minimizing the use of antibiotics. The idea of this project is to reduce environmental impact of whey enormous volumes which are discharged in rivers or on soils, to make the dairy industry more competitive and to solve the big problem of the cheese whey management by its collection, processing and upgrading obtaining high added-value products. Whey is a by-product generated after precipitation and removal of milk casein during cheese manufacturing. Cheese whey is a valorous raw material containing lactose (4.2-4.7% w/w), soluble proteins (0.8 - 1.0% w/w), lipids, mineral salts (4.3 - 10.7% w/w), vitamins. Whey is produced in large amounts, the world whey production in 2008 being over 187 million tones/year, showing a 1-2% annual growth rate (about 9 L per kg of cheese produced). This by-product is an important environmental polluting agent affecting enterprise performances, because of the high volume produced and its high organic matter content, lactose being responsible for the high BOD (biological oxygen demand) and COD (chemical oxygen demand) exhibition in the natural polluted environments. So that whey valorization is imposed. The project attempt to valorize this huge quantity of whey by obtaining high value-added derivatives using whey lactose as bioconversion substrate. Lactic yeast enzymes catalyze the transgalactosylation reaction and hydrolysis of lactose to produce oligosaccharides, glycosides of different degrees of polymerization, used as components of functional foods or nutraceuticals (prebiotics) which pass into the animal colon, where inhibit the pathogenic bacteria and are fermented selectively by beneficial intestinal bacteria (Bifidium sp.) reducing gastrointestinal disorders. Lactic yeast biomass rich in essential amino acids, vitamins, and growth factors represents other research direction, and has an important role to significative improvement of animal weight gain (up to 15%), immune system and quality of products. Therefore, the project is organized in the following main workpackages: 1. Selection of the most efficient and new methods for protein biomass and oligosaccharides obtaining by adapted lactic yeast strains on whey lactose substrate 2. Selection of lactic yeasts adapted and high productively from parental lactic yeast strain by variation of different cultivation parameters for increase synthesis and productivity of single cell biomass and different oligosaccharides 3. Analytical characterization of the parental and selected lactic yeast strains 4. Optimization of the conversion of cheese whey substrate in lactic yeast biomass rich in amino acids and oligosaccharides 5. Characterization of lactic yeast biomass and microelement chelates of amino acids; isolation and characterization of oligosaccharides 6. Elaboration and technological experimentation regarding production of high value-added products 7. Formulation of single cell biomass and production of functional supplements for animal feed samples and testing. The final products will be incorporated in composition of animal fodder being useful for zootechny field.
Acronym: 
OLIGOLAC
Project ID: 
8 076
Start date: 
01-07-2013
Project Duration: 
36months
Project costs: 
1 150 000.00€
Technological Area: 
Precision agriculture technology
Market Area: 
Animal Husbandry

Raising the productivity and competitiveness of European businesses through technology. Boosting national economies on the international market, and strengthening the basis for sustainable prosperity and employment.