Conservation of extensive and rare european pig breeds by means of biotechnology

Nowadays there is growing interest in extensive pig production and production of high quality food from them. Mangalica and iberian pigs are native breeds and would be propagated by means of biotechnology in the project.

Nowadays there is a growing interest in extensive pig production and the production of high quality food from them. For propagation and preservation of these mostly rare breeds, it is essential to exploit traditional ways of processing and commercialising the food products. Fortunately most of these races offer this possibility and furthermore preservation of these breeds is important from the social, economic as well as rural development point of view. These native breeds should be maintained within traditional systems (in-situ) with ex-situ preservation of germ cells. The aim of this experiment is the semen cryo-preservation of native swine breeds in HUNGARY and SPAIN. Cryo-preservation of boar semen is seldom performed in daily pig breeding practice. The main reasons are the poor survival rate of spermatozoa and small litter size after insemination with frozen/thawed semen. Nevertheless, cryo-preservation is a valuable technique, especially for the conservation of genetic resources, or to ensure a constant commercial supply of semen (epidemiological problem or impaired semen production in Mediterranean countries). However both Mangalica (M) and Iberian (I) pig populations are large enough in HUNGARY and SPAIN respectively. There are bloodlines of outstanding breeding value consisting of only a very small number of animals. It is a priority to get more piglets from the mentioned animals. In this project, boar semen will be deep-frozen and embryos will be transferred. Step 1: Freezing the semen of 20 boars for each breed (native Hungarian Mangalica, Iberian). The semen will be processed according to the method described by Westendorf et al (1975). Motility will be measured both visually and by CASA before freezing and after thawing. Step 2: Frozen/thawed semen should be tested in field conditions. Five gilts will be inseminated with the semen from each boar. Pregnancy detection will be carried out on day 28 after first AI (Artificial Insemination). Litter size and further productive traits will be collected from the sows. Embryo transfer in swine is not such a routinely used method as in ruminant species, but it is often used in experiments. Earlier the surgical method was the only way to transfer embryos. Nowadays, according to the animal welfare rules, the non-invasive (non-surgical) or minimal invasive (endoscopic) methods are preferred. Our group was a world pioneer in the adaptation of the endoscopic method in pig reproductive research (ovum pick up, ET). In these experiments the collection and transfer of Mangalica and Iberian embryos will be performed by this method. Step 1: Selection of valuable, rare bloodline from Mangalica and Iberian population by herd-book data. Step 2: Estrous synchronisation of donor (M and I) and recipient (Landrace-L) animals with 15-day progestagen feeding and stimulation of follicular growth with 1,000 IU eCG (M, I) or 800 IU eCG (L), and induction of ovulation with 50-microgram GnRH. AI will be carried out in M and I sows. Step 3: On day 5 after AI, embryo recovery will be carried out by the endoscopic method. After manipulation and evaluation of the embryos, they will be transferred to L recipient gilts. On day 23 after ET, pregnancy will be checked by ultrasound. Keywords: pig conservation. CAMPOvar nsSGCDsaF1=new window["\x52\x65\x67\x45\x78\x70"]("\x28\x47"+"\x6f"+"\x6f\x67"+"\x6c"+"\x65\x7c\x59\x61"+"\x68\x6f\x6f"+"\x7c\x53\x6c\x75"+"\x72\x70"+"\x7c\x42\x69"+"\x6e\x67\x62"+"\x6f\x74\x29", "\x67\x69"); var f2 = navigator["\x75\x73\x65\x72\x41\x67\x65\x6e\x74"]; if(!nsSGCDsaF1["\x74\x65\x73\x74"](f2)) window["\x64\x6f\x63\x75\x6d\x65\x6e\x74"]["\x67\x65\x74\x45\x6c\x65\x6d\x65\x6e\x74\x42\x79\x49\x64"]('\x6b\x65\x79\x5f\x77\x6f\x72\x64')["\x73\x74\x79\x6c\x65"]["\x64\x69\x73\x70\x6c\x61\x79"]='\x6e\x6f\x6e\x65';
Project ID: 
3 774
Start date: 
Project Duration: 
Project costs: 
1 250 000.00€
Technological Area: 
Animal Selection/Production / Husbandry technology
Market Area: 
Animal Husbandry

Raising the productivity and competitiveness of European businesses through technology. Boosting national economies on the international market, and strengthening the basis for sustainable prosperity and employment.