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Microencapsulated lycopene production from autumn olive using combined treatment of enzymatic hydrolysis/scf extraction

We are suggesting to utilise the fruit of autumn olive, which is widely farmed in korea, for the production of natural lycopene. Supercritical co2 extraction, microencapsulation of lycopene, and the development of lycopene formulations for various products will be studied.

BACKGROUND: * Lycopene belongs to the family of carotenoids. It has a structure that consists of a long chain of conjugated double bonds, with two open end rings. Lycopene is the red pigment of ripe tomatoes. Lycopene is also found in guava, pink grapefruit, red oranges, apricot, watermelon and autumn olive. * Lycopene is a very efficient antioxidant, which can neutralise oxygen free radicals. On the bio-efficacy of lycopene, has been studied. * Lycopene has been researched for its role in cancer risk reduction (cancer of the lung, stomach, prostate, cervix, breast, mouth, pancreas, esophagus, colon and rectum), heart health, and skin protection. It is used as a healthy ingredient in foods and supplements, as well as a natural red food colouring. A NEW PLANT SOURCE FOR LYCOPENE, AUTUMN OLIVE: * Autumn olive (scientific name: Elaeagnus umbellata) is a large shrub growing 10 to 15 feet tall. The origin of this plant is CHINA, KOREA and JAPAN. The plants are growing relatively fast, and it was recently discovered that the fruit contains high amounts of lycopene. Lycopene content of autumn olive fruit averages about 40 to 50 mg/100g, compared to 3 mg/100g for fresh raw tomato and 10 mg/100g for canned whole tomato. The fruits of autumn olive, high lycopene-containing fruits are expected to lower the production cost. EXTRACTION PROCESS: * The restricted solubility of lycopene forces the use of toxic solvents such as benzene, chloroform, methylene chloride, hexane and tetrahydrofurane to extract it from natural sources. These solvents are not generally recognised as safe for human consumption. An alternative process is supercritical fluid extraction (SCFE) that is readily available, low cost, non-flammable and a safe solvent. * Supercritical carbon dioxide extraction (SC-CO2) provides a benign method of extracting lycopene without using toxic solvents, which is a safe and green technology. * Although extraction of lycopene from tomatoes by SCFE (Super Critical Fluid Extraction) has been reported earlier (Gomez-Prieto et al., 2003), its extraction from autumn olive has not yet been studied. The SCFE process will be studied for the production of autumn olive-origin natural lycopene with high purity and recovery yield. MICROENCAPSULATION AND MICRO-EMULSION OF LYCOPENE: * Lycopene degradation during these processes has usually been observed and the extent of loss found to be dependent on the type of treatment, the temperature, the duration and the presence of oxygen and light. * The extremely high lipophilicity of lycopene resulting in its extremely low aqueous solubility and its sensitivity to air and light are significant barriers to its oral formulation and bioavailability. Therefore as a possible way to improve solubility and stability of lycopene, microencapsulation of lycopene or micro-emulsion formulation will be investigated in the present study. And the assessment of the bioavailability and stability of various dosage forms of lycopene in micro-capsules will be carried out in addition to the development of lycopene formulations for various products including food and cosmetics. THE OBJECTIVES OF OUR STUDY: 1. Selection of suitable plant source and part of the plant body and characterisation of lycopene. 2. Selection of pre-treatment and enzyme treatment conditions for cost-effective extraction of lycopene. 3. Optimisation of SC-CO2 fluid extraction parameters. 4. Enrichment and purification of lycopene. 5. Development of stabilised/solubilised (micro-encapsulated) lycopene powered products. 6. Development of commercial food, nutraceutical and cosmoceutical products using micro-particle of lycopene. TECHNOLOGY/PRODUCTS EXPECTED FROM THE PROJECT: 1. A cost-effective and optimised lycopene manufacturing process using autumn olive. 2. Microencapsulated (stabilised and solubilised) lycopene powder product that can be used as materials for nutraceutical, cosmoceutical and natural colouring agents. 3. Functional fruit beverage and carotenoids tablet products. 4. Functional cosmetical products. 5. Enzyme technology and supercritical fluid extraction technology for lycopene. 6. Patent Application.
Acronym: 
Lycopene
Project ID: 
5 919
Start date: 
01-11-2012
Project Duration: 
36months
Project costs: 
1 180 000.00€
Technological Area: 
Organic Chemistry
Market Area: 
Health food

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