Elimination of anti-nutritive effects of feed components by enzymes

Development of biotechnological systems to reduce activity
of anti-nutritive factors. As a result nutritional value of
feed and food will increase, market competition will be
improved and excretion of nutrients into environment reduced

Most feedstuffs contain substances which interfere with the digestion and utilisation of nutrients. In protein-rich components factors hampering optimal digestion and utilisation are mainly related to protein structure, antigenicity of protein and so-called Antinutritional Factors (ANFs). In carbohydrate-rich feedstuffs these factors are mainly related to substances such as pectins, lignins and other Non-Starch Polysaccharides (NSPs). The aim of this project is to develop enzymes capable of reducing the activity of factors with antinutritive properties. An example showing that the use of enzymes is a good option to reduce antinutritive effects is the success of the development of the enzyme phytase which breaks down phytic acid. There are three important areas in which protein and carbohydrate-rich products are used: - the animal raw material and feed industry. For this application the nutritional value is the most important criterion. The protein must be highly digestible to obtain a high nutritional value and to minimise the excretion of nitrogen in order to reduce environmental pollution in the form of nitrogen. The carbohydrates must be low viscous (especially for poultry) and the digestibility must be high to obtain a high energetic value. For this area the use of enzymes (mainly ANF-ases but also some NSP-ases) in combination with feed processing technology and feeding systems (wet and dry feeding) are the main interests. - the area of special diets comprising the use of special products with the aim of developing special alternative protein sources in veal calf and piglet diets and for use in diets for pets and fish. These products are generally characterised by a high protein and low carbohydrate content. At present soya protein concentrate and isolate are the main skim milk protein replacers. However, the existing products are not adequate enough for full replacement of skim milk protein, because digestibility is generally lower and the growth and performance is not optimal. The aim is to improve the digestibility of these alternative products using ANF-ases. It is preferred to include the use of enzymes in the production process of the feed component, but addition in the special diets may also be an option. - the human food and specialty feed industry. For this application not only is the nutritional value important but also the functional properties such as solubility and dispersibility. With enzymes the functional properties such as solubility may remain more intact than with processing technologies such as solvent extraction toasting, extrusion and expansion, or they can restore the functionality after such processing. For this area, ANF-ases may be of importance. It is preferred to include the enzymes in the manufacturing process of raw materials but application in the end products may also be a possibility. In biotechnological in-vitro screening tests, enzyme systems are found which break down proteinaceous ANFs very quickly in soya and peas using 40-70% moisture. However, no information is available to what extent the nutritional value is increased due to this treatment. The main activities will firstly be focused on these ANF-ases, but during the project other enzymes will also be involved. Main research items will be identification, development and production of ANF-ases and other enzymes, testing and optimisation of the enzyme systems, evaluation of the enzyme stability in feed and food production and systems and testing the effects on nutritional value of the treated products and functional properties.
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Project costs: 
1 580 000.00€
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Raising the productivity and competitiveness of European businesses through technology. Boosting national economies on the international market, and strengthening the basis for sustainable prosperity and employment.